Explore conservation biology and diversity in high resolution
Often only a single, fragmented reference genome is available for a species, genus, or even family, limiting discovery potential. Single Molecule, Real-Time (SMRT) Sequencing provides the longest reads with uniform coverage and the highest consensus accuracy, generating high-quality reference genomes and contiguous assemblies. These comprehensive assemblies capture undetected SNPs, fully intact genes, and regulatory regions embedded in complex structures that fragmented draft genomes often miss.
Explore more about the value of high-quality reference genomes:
- Application Brief: Large genome whole genome sequencing – Best Practices
- Poster:Korlach, Jonas et al. (2018) High-quality de novo genome assembly and intra-individual mitochondrial instability in the critically endangered kakapo
- Publication: Saarinen, Emily V. et al. (2016) Conservation genetics of an endangered grassland butterfly (Oarisma poweshiek) reveals historically high gene flow despite recent and rapid range loss. Insect Conservation and Diversity
Make direct, evidence-based genome annotations
A single gene may code for a surprising number of proteins, each with a distinct biological function. This is especially true in higher organisms. The PacBio Isoform Sequencing (Iso-Seq) method employs long reads to sequence transcript isoforms of up to 10 kb in their entirety. Surveying this transcript diversity, either broadly or in a targeted fashion, reveals key information about the frequency and types of alternative transcription, improving genome annotation and gene discovery.
Explore more about full-length transcripts with no assembly required:
- Application Brief: Long-read RNA sequencing – Best Practices
- Poster:Concepion, C., et al. (2018) De novo assembly and preliminary annotation of the Schizocardium californicum genome